Wednesday, September 20, 2017

Asiatic Honey Bee (A. cerana) Venom Shows Anti-Fibrinolytic and Anti-Microbial Activity

Anti-fibrinolytic and anti-microbial activities of a serine protease inhibitor from honeybee (Apis cerana) venom

Comp Biochem Physiol C Toxicol Pharmacol. 2017 Sep 13

Bee venom contains a variety of peptide constituents, including low-molecular-weight protease inhibitors. While the putative low-molecular-weight serine protease inhibitor Api m 6 containing a trypsin inhibitor-like cysteine-rich domain was identified from honeybee (Apis mellifera) venom, no anti-fibrinolytic or anti-microbial roles for this inhibitor have been elucidated.

In this study, we identified an Asiatic honeybee (A. cerana) venom serine protease inhibitor (AcVSPI) that was shown to act as a microbial serine protease inhibitor and plasmin inhibitor. AcVSPI was found to consist of a trypsin inhibitor-like domain that displays ten cysteine residues.

Interestingly, the AcVSPI peptide sequence exhibited high similarity to the putative low-molecular-weight serine protease inhibitor Api m 6, which suggests that AcVSPI is an allergen Api m 6-like peptide. Recombinant AcVSPI was expressed in baculovirus-infected insect cells, and it demonstrated inhibitory activity against trypsin, but not chymotrypsin. Additionally, AcVSPI has inhibitory effects against plasmin and microbial serine proteases; however, it does not have any detectable inhibitory effects on thrombin or elastase.

Consistent with these inhibitory effects, AcVSPI inhibited the plasmin-mediated degradation of fibrin to fibrin degradation products. AcVSPI also bound to bacterial and fungal surfaces and exhibited anti-microbial activity against fungi as well as gram-positive and gram-negative bacteria.

These findings demonstrate the anti-fibrinolytic and anti-microbial roles of AcVSPI as a serine protease inhibitor.

Tuesday, September 19, 2017

Bee Venom May Help Treat Neuropathic Pain


Antiallodynic Effects of Bee Venom in an Animal Model of Complex Regional Pain Syndrome Type 1 (CRPS-I)

Toxins 2017, 9(9), 285

Neuropathic pain in a chronic post-ischaemic pain (CPIP) model mimics the symptoms of complex regional pain syndrome type I (CRPS I). The administration of bee venom (BV) has been utilized in Eastern medicine to treat chronic inflammatory diseases accompanying pain. However, the analgesic effect of BV in a CPIP model remains unknown.

The application of a tight-fitting O-ring around the left ankle for a period of 3 h generated CPIP in C57/Bl6 male adult mice. BV (1 mg/kg ; 1, 2, and 3 times) was administered into the SC layer of the hind paw, and the antiallodynic effects were investigated using the von Frey test and by measuring the expression of neurokinin type 1 (NK-1) receptors in dorsal root ganglia (DRG). The administration of BV dose-dependently reduced the pain withdrawal threshold to mechanical stimuli compared with the pre-administration value and with that of the control group. After the development of the CPIP model, the expression of NK-1 receptors in DRG increased and then decreased following the administration of BV.

SC administration of BV results in the attenuation of allodynia in a mouse model of CPIP. The antiallodynic effect was objectively proven through a reduction in the increased expression of NK-1 receptors in DRG

Monday, September 18, 2017

Health Benefits of Manuka Honey as an Essential Constituent for Tissue Regeneration

Curr Drug Metab. 2017 Sep 11

Honey is known for its therapeutic properties from ancient civilizations but only since last few decades its mechanism has been studied on how it causes epithelial regeneration leading to wound and ulcer healing..

In the present review the health perspectives of honey, its chemical composition with special reference to flavonoids, polyphenol composition and other bioactive trace compounds used in tissue regeneration have been highlighted. Honey can inhibit carcinogenesis by moderating with molecular processes of initiation, advancement and progression stage of cancer cells, therefore it is considered a promising anti-cancer agent. Several, well-intentioned characteristics have drawn the attention of researchers to check copious endowed-biological activities of Manuka honey, including antioxidant, antimicrobial and anti-proliferative capacities against cancer cells. Thus, scientists are trying to use Manuka honey in the area of biomedical and tissue engineering to design a template for regeneration.

Naturally derived antibacterial agents, like Manuka honey, contain mixture of compounds, which can influence antibacterial potency. The non-peroxide bacteriostatic properties of Manuka honey have been formerly associated to the presence of methylglyoxal (MGO). The assimilation of MGO as a functional antibacterial additive during designing a tissue template production would explore its properties as a potential agent for manufacturing tissue regeneration template.

The role of glyoxal (GO) and MGO in the bacterial growth inhibition, and in addition to immunomodulatory role, it also enhances wound healing and tissue regeneration. Researchers should step forward to explore the biomedical application, particularly integration into tissue regeneration templates.

Therefore, further studies are fully needed to provide detailed information on active components of Manuka honey and their potential therapeutic efficacy in numerous models of human diseases.

Sunday, September 17, 2017

Research on Honey as Anti-Cancer Agent


New discovery in UAEU research project on cancer-tackling qualities of honey

Researchers at United Arab Emirates University (UAEU) have taken their groundbreaking studies into the role of honey in battling cancer a step further, through new findings that pinpoint its potential impact.

The beneficial effects of Manuka honey — renowned for its anti-bacterial properties and capacity to heal wounds — as an anti-cancer agent were identified in 2013 by a research group headed by Dr. Basel Al-Ramadi, then chair of the Department of Medical Microbiology and Immunology within UAEU’s College of Medicine and Health Sciences (CMHS).

Now further investigation by the team has discovered a particular molecular aspect of breast cancer that this type of honey can combat, paving the way for the development of new understanding about precisely how it can be used to fight one of the world’s most serious diseases, and enhancing UAEU’s reputation for pioneering medical research capable of global impact....

Saturday, September 16, 2017

Propolis Effectively Prevents Chemotherapy-Induced Oral Mucositis

Oncology Nurse Advisor

The combination of propolis and sodium bicarbonate is an effective and well-tolerated therapeutic option to prevent oral mucositis (OM) in patients with breast cancer receiving chemotherapy, according to a study published The European Journal of Cancer Care.

Previous studies exploring the use of propolis, a waxy substance produced by bees that is considered to be a complementary therapy to anticancer agents, for the treatment of esophagitis, stomatitis, oral ulcers, and other conditions achieved mixed results. For this study, researchers investigated the effects of propolis in the prevention of chemotherapy-induced OM in patients with breast cancer.

Friday, September 15, 2017

Charles Mraz Apitherapy Course And Conference (CMACC)


Theory In Practice, A Hands-On Approach

November 10-12 2017

The Redondo Beach Hotel

400 N. Harbor Drive

Redondo Beach, CA 90277

310-376-0746

Thursday, September 14, 2017

Antioxidant and Cytotoxic Properties of Brazilian Propolis

Antioxidant and cytotoxic activity of propolis of Plebeia droryana and Apis mellifera (Hymenoptera, Apidae) from the Brazilian Cerrado biome

Published: September 12, 2017

Propolis is a complex bioactive mixture produced by bees, known to have different biological activities, especially in countries where there is a rich biodiversity of plant species. The objective of this study was to determine the chemical composition and evaluate the antioxidant and cytotoxic properties of Brazilian propolis from the species Plebeia droryana and Apis mellifera found in Mato Grosso do Sul, Brazil.

In the ethanolic extracts of P. droryana propolis (ExEP-P) and A. mellifera (ExEP-A) acids, phenolic compounds, terpenes and tocopherol were identified as major compounds. Both extracts presented antioxidant activity against the 2,2-diphenyl-1-picrylhydrazyl (DPPH) free radical, the maximum activities being 500 μg/mL (ExEP-P) and 300 μg/mL (ExEP-A). However, only ExEP-A was able to inhibit lipid peroxidation induced by the oxidizing agent 2,2'-azobis(2-amidinopropane) dihydrochloride (AAPH), which inhibited oxidative hemolysis and reduced the levels of malondialdehyde (MDA) in human erythrocytes for 4 h of incubation. The extracts also reduced the cell viability of the K562 erythroleukemia tumour line, with a predominance of necrotic death.

Thus, it is concluded that the propolis produced by P. droryana and A. mellifera contain important compounds capable of minimizing the action of oxidizing substances in the organism and reducing the viability of erythroleukemia cells.

Wednesday, September 13, 2017

Manuka Honey Helps Heal Horse Wounds

Manuka honey proves it worth in healing horse wounds, but quality is essential – study
September 13, 2017, Horsetalk.co.nz

The value of manuka honey in helping to heal horse wounds has been shown in an Australian study, but the researchers say its important to use only the highest grades.

The researchers showed that manuka honey can have significant healing properties but not when it has a low Unique Manuka Factor (UMF).

Manuka honey is the only honey graded for antibacterial activity. It is graded against the standard antiseptic phenol.

The UMF scale runs from 0-30 and graded honey will have this on the packaging.

The study team tested whether UMF 20 honey had better effects on healing than UMF 5 honey and generic-store bought honey. They compared the results to healing of untreated wounds treated only with saline as a control.

The researchers also analysed whether the healing was primarily due to manuka honey’s antibacterial effects or because of effects on the cells that help healing.

Professor Andrew Dart, from the University of Sydney School of Veterinary Science, said the research was the most recent of a series of studies investigating the effects of manuka honey on healing of open wounds of horses...

Tuesday, September 12, 2017

Royal jelly – the nobility’s food

Times of Malta

Nature has provided us with several different ‘superfoods’ that have proven to be beneficial to our overall health and fitness. Nevertheless, Royal jelly (RJ) is more than likely the reigning king… or queen of these natural products.

Most people assume that honey is the only bees’ product we collect from the beehive. However, bees also give us RJ, propolis and pollen. Propolis is a resinous substance that the bees produce from tree glue which is mixed with their saliva. Both propolis and pollen are used in alternative medicine. RJ is a thick, milky-white, creamy liquid secreted from glands of the nurse bees, which is extremely nutritious and complex in its chemical composition...

Monday, September 11, 2017

Gwyneth Paltrow Uses Bee Venom Treatment to Get Rid of Her Scars


Gwyneth Paltrow Swears This Strange Treatment Got Rid of Her Scars
Liz Ritter, September 09, 2017

Gwyneth Paltrow's premiere issue of Goop magazine hit yesterday for pre-order and, besides a pretty revealing cover shot of the star, she gets into something she swears by that may have some people—not too surprisingly—scratching their heads: Bee venom injections in her C-section scar.

As reported on by Us, the 44-year-old shared that, "The doctor stings you [with a live bee] like it's an acupuncture needle. I had it done on my cesarean scar… I had some buckling in the scar, and it really evened it out."

Sounds out there but New York dermatologist Adebola Dele-Michael, MD, says bee venom contains hyaluronidase and other substances, which can soften scar tissue and keloids, but stresses that "caution must be taken with this treatment as some people are allergic to bee venom and may develop anaphylaxis when exposed to bee venom."

Sunday, September 10, 2017

Free Amino Acids Profile of Polish and Slovak Honeys

Free amino acids profile of Polish and Slovak honeys based on LC–MS/MS method without the prior derivatisation


Journal of Food Science and Technology, pp 1–8


LC–MS/MS method was applied for determination of free amino acids in honey without derivatisation steps.

Twenty free amino acids including aspartic acid, asparagine, glutamic acid, glutamine, alanine, arginine, glycine, leucine, histidine, hydroxyproline, isoleucine, lysine, methionine, phenylalanine, proline, serine, threonine, tryptophan, valine and ornithine were analyzed in thirty honey samples from Poland and Slovakia.

The analysis covered: acacia, lime, rape, multifloral and forest types of honey. Applied method was characterized to had good sensitivity with limit of detection ranged from 3.0 ng/cm3 for valine to 13.0 ng/cm3 for hydroxyproline.

Average content of proline (main amino acid component in honey) ranged from 151.46 μg/g (rape honey from Slovakia) to 389.66 μg/g for forest honey (honeydew honey) from Poland. In analyzed honeys large quantities of glutamine, glutamic acid, lysine, phenylalanine, asparagine, alanine, and valine were also found.

Saturday, September 09, 2017

Honey Mitigates Radiation-Induced Oral Mucositis in Head and Neck Cancer Patients

Honey Mitigates Radiation-Induced Oral Mucositis in Head and Neck Cancer Patients without Affecting the Tumor Response.

Foods. 2017 Sep 6;6(9). pii: E77

Radiation-induced mucositis is a dose-limiting factor in the effective treatment of head and neck (H amp; N) cancers. The objective of this study was to understand the efficacy of honey in mitigating radiation-induced mucositis and whether it would interfere with tumor control. This was a single-blinded, randomized, controlled study and was carried out in patients with H & N cancer requiring curative radiotherapy (gt;62 Gy (Gray)). The patients meeting the inclusion criteria were randomly assigned to receive either honey ( n = 25) or povidone-iodine (active comparator) ( n = 25) during radiotherapy. Oral mucositis was assessed using the RTOG (Radiation Therapy Oncology Group) grading system before the start, during, and at the end of the treatment by an investigator unaware of the treatment. The results indicate that when compared with the active comparator, honey reduced the radiation-induced oral mucositis, decreased the incidence of intolerable mucositis, treatment breaks, loss of treatment days ( p lt; 0.0001 and lt; 0.0003) and did not affect the radiation-induced tumor response. The clinical observations indicate that honey mitigates the radiation-induced mucositis and does not interfere with tumor cell killing.

Friday, September 08, 2017

Lebanese Propolis Exhibits Significant Cytotoxicity and Anti-Proliferative Activity

Chemical characterization and cytotoxic activity evaluation of Lebanese propolis

Biomed Pharmacother. 2017 Aug 26;95:298-307

Chemical composition, anti-proliferative and proapoptotic activity as well as the effect of various fractions of Lebanese propolis on the cell cycle distribution were evaluated on Jurkat leukemic T-cells, glioblastoma U251 cells, and breast adenocarcinoma MDA-MB-231 cells using cytotoxic assays, flow cytometry as well as western blot analysis.

Liquid chromatography-tandem mass spectrometry (LC-MS/MS) analysis revealed that ferulic acid, chrysin, pinocembrin, galangin are major constituents of the ethanolic crude extract of the Lebanese propolis, while the hexane fraction mostly contains chrysin, pinocembrin, galangin but at similar levels. Furthermore chemical analysis was performed using gas chromatography-mass spectrometry (GC-MS) to identify major compounds in the hexane fraction. Reduction of cell viability was observed in Jurkat cells exposed to the ethanolic crude extract and the hexane fraction, while viability of U251 and MDA-MB-231 cells was only affected upon exposure to the hexane fraction; the other fractions (aqueous phase, methylene chloride, and ethyl acetate) were without effect.

Maximum toxic effect was obtained when Jurkat cells were cultivated with 90μg/ml of both the crude extract and hexane faction. Toxicity started early after 24h of incubation and remained till 72h. Interestingly, the decrease in cell viability was accompanied by a significant increase in p53 protein expression levels and PARP cleavage. Cell cycle distribution showed an increase in the SubG0 fraction in Jurkat, U251 and MDA-MB-231 cells after 24h incubation with the hexane fraction. This increase in SubG0 was further investigated in Jurkat cells by annexinV/PI and showed an increase in the percentage of cells in early and late apoptosis as well as necrosis.

In conclusion, Lebanese propolis exhibited significant cytotoxicity and anti-proliferative activity promising enough that warrant further investigations on the molecular targets and mechanisms of action of Lebanese propolis.

Thursday, September 07, 2017

Manuka Honey Kills Wound Pathogens

Comprehensive In Situ Killing of Six Common Wound Pathogens With Manuka Honey Dressings Using a Modified AATCC-TM100

Wounds. 2017 Jul 26. pii: WNDS20170726-3

OBJECTIVE:

While Manuka honey in vitro is strongly antimicrobial, there have been, to the best of the authors' knowledge, no studies showing that dressings impregnated with Manuka honey can kill organisms in the dressing itself.

MATERIALS AND METHODS:

The investigators used the American Association of Textile Chemists and Colorists' 100 test methodology to compare honey-impregnated dressings with control dressings (without honey) on the ability to kill common wound pathogens. Organisms were chosen after a review of the causal organisms found in actual wound infections over a 12-month period in a busy outpatient wound clinic.

RESULTS:

Even when the dressings were challenged daily with further inoculated organisms, > 5-log reductions were routinely noted across a range of pathogens, including multiple drug-resistant species using dressings containing Manuka honey relative to the control.

CONCLUSIONS:

The results presented herein show that when well-characterized medical-grade Manuka honey is used in dressings (ie, a minimum of 400 mg methylglyoxal/kg) these dressings can comprehensively kill common wound pathogens associated with infected wounds

Wednesday, September 06, 2017

Manuka Honey Used to Treat Leg Wounds on Horses


This is how fast manuka honey can heal wounds

Manuka honey with a high UMF rating is significantly more effective than examples with a low rating, or regular honey, in improving wound healing, new research shows.

Researchers at the University of Sydney compared the effectiveness of manuka honey of different UMF (unique manuka factor) ratings (ranging from 5 to 20 depending on antibacterial activity) and ordinary, store-bought honey in healing large, bloody wounds on horses' legs.

They said the results were "quite remarkable" and applicable to humans.

"I have been doing wound-healing studies for many years and if you look across the board there are very few, if any, compounds that have a consistent repeatable effect," Sydney University equine surgical specialist Andrew Dart said...